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mrfp1 reporter plasmid  (Addgene inc)


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    Addgene inc mrfp1 reporter plasmid
    ( a ) Control of mCherry repression using a LacI-LOVdeg fusion. ( b ) mCherry expression in response to light exposure for strains with LacI-LOVdeg compared to IPTG induction (**p<0.001, two-tailed unpaired t -test). ( c ) Schematic of SoxS-based CRISPRa activation with a LOVdeg tag appended to the MCP-SoxS activator. ( d ) CRISPRa control of <t>mRFP1</t> expression in response to light (***p<0.0001, two-tailed unpaired t -test). ( e ) Schematic of the LOVdeg tag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. ( f ) Chloramphenicol sensitivity tests. Wild-type cells (BW25113) are compared to a Δ acrB (BW25113 Δ acrB ) strain, Δ acrB complemented with AcrB-LOVdeg (ΔacrB + AcrB-LOVdeg) exposed to light or kept in the dark, and Δ acrB strain complemented with an IPTG-inducible AcrB (Δ acrB + AcrB). No IPTG was added to Δ acrB + AcrB or Δ acrB + AcrB-LOVdeg. ( g ) OD 600 of strains shown in ( f ) at 2.5 μg/mL chloramphenicol (**p<0.001; *p<0.05; ns, not significant; two-tailed unpaired t -test). Error bars show standard deviation around the mean (n = 3 biological replicates).
    Mrfp1 Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mrfp1+reporter+plasmid/pmc10945698-247-1-8?v=Addgene+inc
    Average 92 stars, based on 6 article reviews
    mrfp1 reporter plasmid - by Bioz Stars, 2026-07
    92/100 stars

    Images

    1) Product Images from "Light-inducible protein degradation in E. coli with the LOVdeg tag"

    Article Title: Light-inducible protein degradation in E. coli with the LOVdeg tag

    Journal: eLife

    doi: 10.7554/eLife.87303

    ( a ) Control of mCherry repression using a LacI-LOVdeg fusion. ( b ) mCherry expression in response to light exposure for strains with LacI-LOVdeg compared to IPTG induction (**p<0.001, two-tailed unpaired t -test). ( c ) Schematic of SoxS-based CRISPRa activation with a LOVdeg tag appended to the MCP-SoxS activator. ( d ) CRISPRa control of mRFP1 expression in response to light (***p<0.0001, two-tailed unpaired t -test). ( e ) Schematic of the LOVdeg tag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. ( f ) Chloramphenicol sensitivity tests. Wild-type cells (BW25113) are compared to a Δ acrB (BW25113 Δ acrB ) strain, Δ acrB complemented with AcrB-LOVdeg (ΔacrB + AcrB-LOVdeg) exposed to light or kept in the dark, and Δ acrB strain complemented with an IPTG-inducible AcrB (Δ acrB + AcrB). No IPTG was added to Δ acrB + AcrB or Δ acrB + AcrB-LOVdeg. ( g ) OD 600 of strains shown in ( f ) at 2.5 μg/mL chloramphenicol (**p<0.001; *p<0.05; ns, not significant; two-tailed unpaired t -test). Error bars show standard deviation around the mean (n = 3 biological replicates).
    Figure Legend Snippet: ( a ) Control of mCherry repression using a LacI-LOVdeg fusion. ( b ) mCherry expression in response to light exposure for strains with LacI-LOVdeg compared to IPTG induction (**p<0.001, two-tailed unpaired t -test). ( c ) Schematic of SoxS-based CRISPRa activation with a LOVdeg tag appended to the MCP-SoxS activator. ( d ) CRISPRa control of mRFP1 expression in response to light (***p<0.0001, two-tailed unpaired t -test). ( e ) Schematic of the LOVdeg tag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. ( f ) Chloramphenicol sensitivity tests. Wild-type cells (BW25113) are compared to a Δ acrB (BW25113 Δ acrB ) strain, Δ acrB complemented with AcrB-LOVdeg (ΔacrB + AcrB-LOVdeg) exposed to light or kept in the dark, and Δ acrB strain complemented with an IPTG-inducible AcrB (Δ acrB + AcrB). No IPTG was added to Δ acrB + AcrB or Δ acrB + AcrB-LOVdeg. ( g ) OD 600 of strains shown in ( f ) at 2.5 μg/mL chloramphenicol (**p<0.001; *p<0.05; ns, not significant; two-tailed unpaired t -test). Error bars show standard deviation around the mean (n = 3 biological replicates).

    Techniques Used: Control, Expressing, Two Tailed Test, Activation Assay, Membrane, Standard Deviation

    ( a ) Genetic constructs of the original SoxS-CRISPRa from Dong et al., where the activator is anhydrotetracycline (aTc) inducible. However, aTc is light sensitive, thus we replaced the TetR/P tet portion with the IPTG-inducible LacI/P trc . ( b ) Comparison of the CRISPRa activity of the original construct and the new P trc promoter construct with and without a gRNA targeting the promoter of mRFP1. Error bars show standard deviation around the mean (n = 3 biological replicates).
    Figure Legend Snippet: ( a ) Genetic constructs of the original SoxS-CRISPRa from Dong et al., where the activator is anhydrotetracycline (aTc) inducible. However, aTc is light sensitive, thus we replaced the TetR/P tet portion with the IPTG-inducible LacI/P trc . ( b ) Comparison of the CRISPRa activity of the original construct and the new P trc promoter construct with and without a gRNA targeting the promoter of mRFP1. Error bars show standard deviation around the mean (n = 3 biological replicates).

    Techniques Used: Construct, Comparison, Activity Assay, Standard Deviation



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    mrfp1 reporter plasmid  (Addgene inc)
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    Addgene inc mrfp1 reporter plasmid
    ( a ) Control of mCherry repression using a LacI-LOVdeg fusion. ( b ) mCherry expression in response to light exposure for strains with LacI-LOVdeg compared to IPTG induction (**p<0.001, two-tailed unpaired t -test). ( c ) Schematic of SoxS-based CRISPRa activation with a LOVdeg tag appended to the MCP-SoxS activator. ( d ) CRISPRa control of <t>mRFP1</t> expression in response to light (***p<0.0001, two-tailed unpaired t -test). ( e ) Schematic of the LOVdeg tag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. ( f ) Chloramphenicol sensitivity tests. Wild-type cells (BW25113) are compared to a Δ acrB (BW25113 Δ acrB ) strain, Δ acrB complemented with AcrB-LOVdeg (ΔacrB + AcrB-LOVdeg) exposed to light or kept in the dark, and Δ acrB strain complemented with an IPTG-inducible AcrB (Δ acrB + AcrB). No IPTG was added to Δ acrB + AcrB or Δ acrB + AcrB-LOVdeg. ( g ) OD 600 of strains shown in ( f ) at 2.5 μg/mL chloramphenicol (**p<0.001; *p<0.05; ns, not significant; two-tailed unpaired t -test). Error bars show standard deviation around the mean (n = 3 biological replicates).
    Mrfp1 Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mrfp1+reporter+plasmid/pmc10945698-247-1-8?v=Addgene+inc
    Average 92 stars, based on 1 article reviews
    mrfp1 reporter plasmid - by Bioz Stars, 2026-07
    92/100 stars
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    mrfp1 nrf2 reporter  (Addgene inc)
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    Addgene inc mrfp1 nrf2 reporter
    ( a ) Control of mCherry repression using a LacI-LOVdeg fusion. ( b ) mCherry expression in response to light exposure for strains with LacI-LOVdeg compared to IPTG induction (**p<0.001, two-tailed unpaired t -test). ( c ) Schematic of SoxS-based CRISPRa activation with a LOVdeg tag appended to the MCP-SoxS activator. ( d ) CRISPRa control of <t>mRFP1</t> expression in response to light (***p<0.0001, two-tailed unpaired t -test). ( e ) Schematic of the LOVdeg tag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. ( f ) Chloramphenicol sensitivity tests. Wild-type cells (BW25113) are compared to a Δ acrB (BW25113 Δ acrB ) strain, Δ acrB complemented with AcrB-LOVdeg (ΔacrB + AcrB-LOVdeg) exposed to light or kept in the dark, and Δ acrB strain complemented with an IPTG-inducible AcrB (Δ acrB + AcrB). No IPTG was added to Δ acrB + AcrB or Δ acrB + AcrB-LOVdeg. ( g ) OD 600 of strains shown in ( f ) at 2.5 μg/mL chloramphenicol (**p<0.001; *p<0.05; ns, not significant; two-tailed unpaired t -test). Error bars show standard deviation around the mean (n = 3 biological replicates).
    Mrfp1 Nrf2 Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mrfp1+reporter+plasmid/pmc07315801-592-2-4?v=Addgene+inc
    Average 91 stars, based on 1 article reviews
    mrfp1 nrf2 reporter - by Bioz Stars, 2026-07
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Control of mCherry repression using a LacI-LOVdeg fusion. ( b ) mCherry expression in response to light exposure for strains with LacI-LOVdeg compared to IPTG induction (**p<0.001, two-tailed unpaired t -test). ( c ) Schematic of SoxS-based CRISPRa activation with a LOVdeg tag appended to the MCP-SoxS activator. ( d ) CRISPRa control of mRFP1 expression in response to light (***p<0.0001, two-tailed unpaired t -test). ( e ) Schematic of the LOVdeg tag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. ( f ) Chloramphenicol sensitivity tests. Wild-type cells (BW25113) are compared to a Δ acrB (BW25113 Δ acrB ) strain, Δ acrB complemented with AcrB-LOVdeg (ΔacrB + AcrB-LOVdeg) exposed to light or kept in the dark, and Δ acrB strain complemented with an IPTG-inducible AcrB (Δ acrB + AcrB). No IPTG was added to Δ acrB + AcrB or Δ acrB + AcrB-LOVdeg. ( g ) OD 600 of strains shown in ( f ) at 2.5 μg/mL chloramphenicol (**p<0.001; *p<0.05; ns, not significant; two-tailed unpaired t -test). Error bars show standard deviation around the mean (n = 3 biological replicates).

    Journal: eLife

    Article Title: Light-inducible protein degradation in E. coli with the LOVdeg tag

    doi: 10.7554/eLife.87303

    Figure Lengend Snippet: ( a ) Control of mCherry repression using a LacI-LOVdeg fusion. ( b ) mCherry expression in response to light exposure for strains with LacI-LOVdeg compared to IPTG induction (**p<0.001, two-tailed unpaired t -test). ( c ) Schematic of SoxS-based CRISPRa activation with a LOVdeg tag appended to the MCP-SoxS activator. ( d ) CRISPRa control of mRFP1 expression in response to light (***p<0.0001, two-tailed unpaired t -test). ( e ) Schematic of the LOVdeg tag appended to AcrB of the AcrAB-TolC efflux pump. IM, inner membrane; OM, outer membrane. ( f ) Chloramphenicol sensitivity tests. Wild-type cells (BW25113) are compared to a Δ acrB (BW25113 Δ acrB ) strain, Δ acrB complemented with AcrB-LOVdeg (ΔacrB + AcrB-LOVdeg) exposed to light or kept in the dark, and Δ acrB strain complemented with an IPTG-inducible AcrB (Δ acrB + AcrB). No IPTG was added to Δ acrB + AcrB or Δ acrB + AcrB-LOVdeg. ( g ) OD 600 of strains shown in ( f ) at 2.5 μg/mL chloramphenicol (**p<0.001; *p<0.05; ns, not significant; two-tailed unpaired t -test). Error bars show standard deviation around the mean (n = 3 biological replicates).

    Article Snippet: The mRFP1 reporter plasmid was derived from pJF076Sa (Addgene #113322) by replacing the ampicillin resistance gene with a kanamycin resistance gene from the BglBrick library.

    Techniques: Control, Expressing, Two Tailed Test, Activation Assay, Membrane, Standard Deviation

    ( a ) Genetic constructs of the original SoxS-CRISPRa from Dong et al., where the activator is anhydrotetracycline (aTc) inducible. However, aTc is light sensitive, thus we replaced the TetR/P tet portion with the IPTG-inducible LacI/P trc . ( b ) Comparison of the CRISPRa activity of the original construct and the new P trc promoter construct with and without a gRNA targeting the promoter of mRFP1. Error bars show standard deviation around the mean (n = 3 biological replicates).

    Journal: eLife

    Article Title: Light-inducible protein degradation in E. coli with the LOVdeg tag

    doi: 10.7554/eLife.87303

    Figure Lengend Snippet: ( a ) Genetic constructs of the original SoxS-CRISPRa from Dong et al., where the activator is anhydrotetracycline (aTc) inducible. However, aTc is light sensitive, thus we replaced the TetR/P tet portion with the IPTG-inducible LacI/P trc . ( b ) Comparison of the CRISPRa activity of the original construct and the new P trc promoter construct with and without a gRNA targeting the promoter of mRFP1. Error bars show standard deviation around the mean (n = 3 biological replicates).

    Article Snippet: The mRFP1 reporter plasmid was derived from pJF076Sa (Addgene #113322) by replacing the ampicillin resistance gene with a kanamycin resistance gene from the BglBrick library.

    Techniques: Construct, Comparison, Activity Assay, Standard Deviation